US 12,338,482 B2
Ultralow concentration sensing of bio-matter with perovskite nickelate devices and arrays
Haitian Zhang, Lafayette, IN (US); Fan Zuo, Terre Haute, IN (US); Feiran Li, West Lafayette, IN (US); Jong Hyun Choi, West Lafayette, IN (US); and Shriram Ramanathan, West Lafayette, IN (US)
Assigned to Purdue Research Foundation, West Lafayette, IN (US)
Appl. No. 17/311,707
Filed by Purdue Research Foundation, West Lafayette, IN (US)
PCT Filed Dec. 10, 2019, PCT No. PCT/US2019/065333
§ 371(c)(1), (2) Date Jun. 8, 2021,
PCT Pub. No. WO2020/131475, PCT Pub. Date Jun. 25, 2020.
Claims priority of provisional application 62/782,068, filed on Dec. 19, 2018.
Prior Publication US 2022/0025425 A1, Jan. 27, 2022
Int. Cl. C12Q 1/00 (2006.01); A61B 5/145 (2006.01); A61B 5/1486 (2006.01); G01N 33/487 (2006.01)
CPC C12Q 1/005 (2013.01) [A61B 5/14532 (2013.01); A61B 5/1486 (2013.01); C12Q 1/006 (2013.01); G01N 33/48707 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method of detecting a biomarker in a biological fluid, comprising:
(i) providing a device comprising the following components:
a perovskite nickelate film comprising RNiO3, wherein said perovskite nickelate film is configured as a lattice or with micro-fluidic channels, wherein R is selected from the group consisting of Sm, Nd, Eu, Gd, Dy, Y, Lu, Pr, and La; and
an enzyme horseradish peroxidase (HRP) for detection of the biomarker dopamine conjugated to a conductive material, wherein said conductive material is deposited on said perovskite nickelate film, wherein said enzyme facilitates hydrogen transfer from said biomarker to said perovskite nickelate film and reduces conductivity at the interface between the perovskite nickelate film and said biomarker;
(ii) measuring the resistance reading R0 between the device and the conductive material;
(iii) immersing the device in the biological fluid, wherein the biological fluid is cerebrospinal fluid;
(iv) measuring the resistance reading R between the device and the conductive material after the immersing step; and
(v) determining a resistance ratio R/R0 using the resistance readings of step (iv) and step (ii), respectively, whereupon the biomarker dopamine present in the cerebrospinal fluid is identified when the resistance ratio R/R0 is greater than 1.