	US 12,338,469 B2
	Proteases having improved enzyme stability in washing and cleaning agents III
Nina Mussmann, Willich (DE); Susanne Wieland, Zons/Dormagen (DE); Christian Degering, Erkrath (DE); Layla Fernandez, Cologne (DE); Sabine Griemert, Monheim am Rhein (DE); Barbara Peiffer, Rommerskirchen (DE); Michael Puls, Monheim am Rhein (DE); and Inken Prueser, Duesseldorf (DE)
Assigned to HENKEL AG & CO. KGAA, Duesseldorf (DE)
Appl. No. 17/606,796
Filed by Henkel AG & Co. KGaA, Duesseldorf (DE)
PCT Filed Apr. 14, 2020, PCT No. PCT/EP2020/060385 § 371(c)(1), (2) Date Oct. 27, 2021, PCT Pub. No. WO2020/221579, PCT Pub. Date Nov. 5, 2020.
Claims priority of application No. 10 2019 111 057.2 (DE), filed on Apr. 29, 2019.
Prior Publication US 2022/0204960 A1, Jun. 30, 2022
Int. Cl. C12N 9/54 (2006.01); C11D 3/386 (2006.01)

CPC C12N 9/54 (2013.01) [C11D 3/38618 (2013.01); C11D 2111/12 (2024.01); C11D 2111/14 (2024.01); C12Y 304/21062 (2013.01)]

9 Claims

1. A protease comprising:

an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1;

wherein, the amino acid sequence contains:

(i) amino acid substitutions at positions 9, 144, 252, and 271; and

(ii) at least one amino acid substitution at positions 53, 120, 133, 149, 189, 211, and 274; and/or

(iii) at least one amino acid substitution, selected from the group consisting of 130Q, 130T, 130V, 130R, 131H, 159S, 162E, 162S, 172E, 189T, and combinations thereof.