US 12,338,467 B2
Variant maltohexaose-forming α-amylase variants
Luis G Cascao-Pereira, Redwood City, CA (US); David A. Estell, San Mateo, CA (US); Marc Anton Bernhard Kolkman, Oegstgeest (NL); and Harm Mulder, Voorhout (NL)
Assigned to DANISCO US INC., Palo Alto, CA (US)
Filed by DANISCO US INC., Palo Alto, CA (US)
Filed on Jan. 5, 2024, as Appl. No. 18/405,674.
Application 18/405,674 is a continuation of application No. 17/373,307, filed on Jul. 12, 2021, granted, now 11,898,177.
Application 17/373,307 is a continuation of application No. 16/572,804, filed on Sep. 17, 2019, abandoned.
Application 16/572,804 is a continuation of application No. 15/160,624, filed on May 20, 2016, granted, now 10,457,924, issued on Oct. 29, 2019.
Application 15/160,624 is a continuation of application No. 14/354,425, abandoned, previously published as PCT/US2012/062209, filed on Oct. 26, 2012.
Claims priority of provisional application 61/668,359, filed on Jul. 5, 2012.
Claims priority of provisional application 61/552,910, filed on Oct. 28, 2011.
Prior Publication US 2024/0327814 A1, Oct. 3, 2024
Int. Cl. C12N 9/28 (2006.01); A21D 8/04 (2006.01); C11D 3/386 (2006.01); C12P 7/06 (2006.01); C12P 19/14 (2006.01); D06L 1/14 (2006.01); D06L 4/40 (2017.01)
CPC C12N 9/2417 (2013.01) [A21D 8/042 (2013.01); C11D 3/386 (2013.01); C12Y 302/01001 (2013.01); D06L 1/14 (2013.01); D06L 4/40 (2017.01)] 8 Claims
 
1. A variant α-amylase polypeptide derived from a parental α-amylase polypeptide, comprising at least two combinable mutations at productive amino acid positions; wherein the mutations are at position Q169 and E391, wherein the variant has at least 90% amino acid sequence identity with SEQ ID NO: 3.