	US 12,338,456 B2
	Method for increasing the specific production rate of eukaryotic cells
Oliver Popp, Penzberg (DE)
Assigned to HOFFMANN-LA ROCHE INC., Little Falls, NJ (US)
Filed by Hoffmann-La Roche Inc., Little Falls, NJ (US)
Filed on Nov. 22, 2022, as Appl. No. 17/992,039.
Application 17/992,039 is a division of application No. 16/414,500, filed on May 16, 2019, abandoned.
Application 16/414,500 is a continuation of application No. 15/428,279, filed on Feb. 9, 2017, granted, now 10,336,983, issued on Jul. 2, 2019.
Application 15/428,279 is a continuation of application No. PCT/EP2015/067733, filed on Jul. 31, 2015.
Claims priority of application No. 14180586 (EP), filed on Aug. 11, 2014.
Prior Publication US 2023/0081499 A1, Mar. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 21/00 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01)

CPC C12N 5/0018 (2013.01) [C12N 5/0025 (2013.01); C12N 5/0682 (2013.01); C12N 2500/32 (2013.01); C12N 2500/33 (2013.01); C12N 2501/998 (2013.01); C12N 2510/02 (2013.01)]

11 Claims

1. A method for producing an exogenous polypeptide in a Chinese Hamster Ovary (CHO) cell, wherein the CHO cell expresses a nucleic acid encoding the exogenous polypeptide, the method comprising culturing the CHO cell in a culture medium comprising meta-tyrosine, wherein the meta-tyrosine in the culture medium is at a concentration of from 0.2 mM to 0.7 mM.