	US 12,338,446 B2
	Plants having increased tolerance to herbicides
Raphael Aponte, Mannheim (DE); Stefan Tresch, Ludwigshafen (DE); Dario Massa, Limburgerhof (DE); Tobias Seiser, Mannheim (DE); and Thomas Mietzner, Annweiler (DE)
Assigned to BASF AGRO B.V., Arnhem (NL)
Filed by BASF AGRO B.V., Arnhem (NL)
Filed on Jun. 30, 2022, as Appl. No. 17/855,164.
Application 17/855,164 is a continuation of application No. 15/736,423, abandoned, previously published as PCT/IB2016/053512, filed on Jun. 15, 2016.
Claims priority of application No. 15172587 (EP), filed on Jun. 17, 2015.
Prior Publication US 2023/0036177 A1, Feb. 2, 2023
Int. Cl. C12N 15/82 (2006.01); C12N 9/02 (2006.01)

CPC C12N 15/8274 (2013.01) [C12N 9/001 (2013.01); C12Y 103/03004 (2013.01)]

3 Claims

1. A method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of:

a) providing, at said site, a plant that comprises a nucleic acid encoding a protoporphyrinogen oxidase (PPO), wherein expression of the nucleic acid in the plant results in increased tolerance to 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3-oxo-4-(prop-2-ynyl)-3,4-dihydro-2H-benzo[b][1,4]oxazin-6-yl)-1,3,5-triazinane-2,4-dione as compared to a wild type control plant, and wherein the encoded PPO comprises the amino acid sequence of SEQ ID NO: 184; and

b) applying to said site an effective amount of 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3-oxo-4-(prop-2-ynyl)-3,4-dihydro-2H-benzo[b][1,4]oxazin-6-yl)-1,3,5-triazinane-2,4-dione.