US 12,338,429 B1
Devices, systems, and methods for rehydration of microorganisms to enhance agricultural yields
Bruce H. Caldwell, Columbus, OH (US); Jane Patterson Fife, Powell, OH (US); Rebecca Williams-Wagner, Lewis Center, OH (US); Matt Sells, Dayton, OH (US); and Chris Koenig, Columbus, OH (US)
Assigned to 3Bar Biologics, Inc., Columbus, OH (US)
Filed by 3Bar Biologics, Inc., Columbus, OH (US)
Filed on Feb. 2, 2024, as Appl. No. 18/431,281.
Int. Cl. C12N 1/04 (2006.01); A01N 63/20 (2020.01); A01N 63/30 (2020.01); C12M 1/00 (2006.01); C12N 1/38 (2006.01); C05F 11/08 (2006.01)
CPC C12N 1/04 (2013.01) [A01N 63/20 (2020.01); A01N 63/30 (2020.01); C12M 23/32 (2013.01); C12M 23/34 (2013.01); C12N 1/38 (2013.01)] 12 Claims
OG exemplary drawing
 
1. An apparatus for delivering viable microorganisms in an agricultural applicator, the apparatus comprising:
a first container including a stabilized microbial culture therein;
a second container including an aqueous rehydration solution disposed therein; and
a disruptable dividing member separating the stabilized microbial culture in the first container from the aqueous rehydration solution in the second container,
wherein disrupting the dividing member forms a flow path between the first container and the second container to allow contact between the stabilized microbial culture and the aqueous rehydration solution, and
wherein contact between the stabilized microbial culture and the aqueous rehydration solution re-initiates lag phase cellular activity of the viable microorganisms.