	US 12,338,427 B2
	Distributed systems for the efficient production and use of microbe-based compositions
Sean Farmer, Ft. Lauderdale, FL (US); Paul S. Zorner, Encinitas, CA (US); Ken Alibek, Solon, OH (US); Kent Adams, Oro Valley, AZ (US); and Tyler Dixon, Madison, OH (US)
Assigned to LOCUS SOLUTIONS IPCO, LLC, Solon, OH (US)
Filed by Locus Solutions IPCo, LLC, Solon, OH (US)
Filed on Apr. 5, 2024, as Appl. No. 18/628,247.
Application 18/628,247 is a continuation of application No. 18/447,387, filed on Aug. 10, 2023, granted, now 11,959,062.
Application 18/447,387 is a continuation of application No. 16/331,438, granted, now 11,760,969, issued on Sep. 19, 2023, previously published as PCT/US2017/050711, filed on Sep. 8, 2017.
Claims priority of provisional application 62/385,057, filed on Sep. 8, 2016.
Prior Publication US 2024/0271076 A1, Aug. 15, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/44 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01); C12N 1/14 (2006.01); C12N 1/16 (2006.01); C12N 1/20 (2006.01); C12P 7/64 (2022.01)

CPC C12M 41/26 (2013.01) [C12M 23/44 (2013.01); C12M 23/52 (2013.01); C12M 23/58 (2013.01); C12M 41/12 (2013.01); C12N 1/14 (2013.01); C12N 1/16 (2013.01); C12N 1/20 (2013.01); C12P 7/64 (2013.01); C12P 19/44 (2013.01); Y02A 40/10 (2018.01)]

7 Claims

1. A method for improving water flow through soil and/or improving aeration of soil wherein said method comprises applying to the soil a microbe-based product comprising microbes, a broth in which said microbes were grown, and a biosurfactant produced by said microbes; wherein said method further comprises growing the microbes in a microbe growth facility, harvesting said microbes, said broth and said biosurfactant; creating the microbe-based product comprising the harvested microbes, broth and biosurfactant; transporting the microbe-based product to a location for use; and applying the product to the soil;

wherein the biosurfactant is selected from the group consisting of rhamnolipids, sophorolipids (SLP) and mannosylerythritol lipids (MEL); and

wherein said microbes are selected from the group consisting of Pseudomonas aeruginosa, Starmerella bombicola, Pichia anomala, Wickerhamomyces anomalus, Trichoderma harzianum, Bacillus amyloliquefaciens, Bacillus subtilis, and Pseudozyma aphidis.