US 12,338,424 B2
Cell structure connection method and connection support device
Kumika Matsubayashi, Fukuoka (JP); and Yasuto Kishii, Tokyo (JP)
Assigned to CYFUSE BIOMEDICAL K.K., Tokyo (JP)
Appl. No. 17/046,264
Filed by CYFUSE BIOMEDICAL K.K., Tokyo (JP)
PCT Filed Apr. 9, 2018, PCT No. PCT/JP2018/014891
§ 371(c)(1), (2) Date Oct. 8, 2020,
PCT Pub. No. WO2019/198124, PCT Pub. Date Oct. 17, 2019.
Prior Publication US 2021/0079333 A1, Mar. 18, 2021
Int. Cl. C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12M 1/04 (2006.01); C12M 1/26 (2006.01); C12M 3/00 (2006.01); C12N 5/00 (2006.01)
CPC C12M 25/10 (2013.01) [C12M 21/08 (2013.01); C12M 23/06 (2013.01); C12M 23/24 (2013.01); C12M 23/44 (2013.01); C12M 23/50 (2013.01); C12M 33/00 (2013.01); C12N 5/0068 (2013.01); C12N 2533/30 (2013.01)] 14 Claims
 
1. A connection support device capable of connecting two or more tubular cell structures, each of the tubular cell structures having an inner hollow portion, the connection support device comprising:
a rod-shaped member insertable into the hollow portion of said two or more tubular cell structures, the rod-shaped member including a circular cross section having an outer diameter, wherein said outer diameter is closely contactable to inner surfaces of the tubular cell structures by shrinkage of the tubular cell structures, and said rod-shaped member having a total length longer than a sum of respective lengths of the two or more tubular cell structures, wherein the rod-shaped member is made of a material with oxygen permeability;
a presser device including a clamp portion capable of being fixed onto the rod-shaped member by clamping an outer peripheral surface of the rod-shaped member with the clamp portion to fit an inner surface of the clamp portion to the outer peripheral surface of the rod-shaped member; and
a movement stopping feature positioned to be contacted with one end of the two or more tubular cell structures, and being capable of preventing said tubular structures from moving on said rod-shaped member,
wherein when the rod-shaped member is inserted into the two or more tubular cell structures in a state where ends of the two or more tubular cell structures contact with each other, the clamp portion makes contact with a surface of a first end of the two or more tubular cell structures and the movement stopping feature makes contact with a surface of a second end opposite to the first end of the two or more tubular cell structures.