| CPC C12M 21/08 (2013.01) [C12M 23/16 (2013.01)] | 5 Claims |
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1. A three-dimensional biomimetic chip for simulating an endometrium, the three-dimensional biomimetic chip comprising a plurality of chambers, and a plurality of posts, which are arranged between the plurality of chambers,
wherein the plurality of chambers comprises a third cell culture chamber, a first culture medium chamber, a second cell culture chamber, a first cell culture chamber, and a second culture medium chamber,
wherein the first cell culture chamber comprises a first culture channel in which uterine vascular endothelial cells are cultured;
wherein the second cell culture chamber comprises a second culture channel that communicates with the first culture channel and in which endometrial stromal fibroblasts are cultured;
wherein the first culture medium chamber comprises a first culture medium channel that communicates with the second culture channel, into which a culture medium is injected, and in which endometrial epithelial cells are cultured,
wherein the third cell culture chamber comprises a third culture channel that communicates with the first culture medium channel, and in which endometrial stromal fibroblast are cultured, and
wherein the second culture medium chamber comprises a second culture medium channel that communicates with the first culture channel, and into which a culture medium is injected,
wherein the third culture channel and the second culture medium channel are each the outermost channels among the plurality of channels comprised in the plurality of chambers,
wherein the second culture channel and the third culture channel are positioned on one side of the first culture channel in one direction, and growth of blood vessels formed by uterine vascular endothelial cells in the first culture channel is oriented in the one direction,
wherein the plurality of the posts comprises:
a plurality of first posts arranged along a boundary between the first culture channel and the second culture channel,
a plurality of second posts arranged along a boundary between the second culture channel and the first culture medium channel, and
a plurality of third posts arranged along a boundary between the first culture medium channel and the third culture channel,
wherein each of the plurality of posts comprises a polygonal prism shape comprising one curved surface that curves toward the interior of adjacent channel, and
wherein curved surfaces of the plurality of second posts and the plurality of third posts face in opposite directions, and embryos injected into the first culture medium channel roll on the curved surfaces of the plurality of second posts and the plurality of third posts.
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