US 12,338,416 B2
Fabric and home care products with cold water protease
Philip Frank Souter, Northumberland (GB); Glenn Steven Ward, Newcastle-upon Tyne (GB); Viktor Yuryevich Alekseyev, Palo Alto, CA (US); Joshua Roy Basler, Palo Alto, CA (US); Luis Gustavo Cascao-Pereira, Redwood City, CA (US); David A Estell, San Francisco, CA (US); Ayrookaran J Poulose, Belmont, CA (US); James T Kellis, Jr., Woodside, CA (US); Alexander Pisarchik, Belmont, CA (US); and Daniel Torres-Pazmino, KA Leiden (NL)
Assigned to The Procter & Gamble Company, Cincinnati, OH (US)
Filed by The Procter & Gamble Company, Cincinnati, OH (US)
Filed on Nov. 18, 2020, as Appl. No. 16/951,344.
Application 16/951,344 is a continuation of application No. 15/839,900, filed on Dec. 13, 2017, granted, now 10,900,000.
Application 15/839,900 is a continuation of application No. 14/205,835, filed on Mar. 12, 2014, abandoned.
Application 14/205,835 is a continuation of application No. 12/963,681, filed on Dec. 9, 2010, granted, now 8,785,171, issued on Jul. 22, 2014.
Claims priority of provisional application 61/392,175, filed on Oct. 12, 2010.
Claims priority of provisional application 61/267,878, filed on Dec. 9, 2009.
Prior Publication US 2021/0071113 A1, Mar. 11, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C11D 3/386 (2006.01)
CPC C11D 3/38618 (2013.01) [C11D 3/38609 (2013.01); C11D 3/38681 (2013.01)] 6 Claims
 
1. A detergent composition, comprising a variant of a parent protease, wherein the parent protease comprises the amino sequence of SEQ ID NO: 2, the protease variant comprising a total of eight, nine, 10, 11, 12, 13, 14, or 15 mutations relative to the amino acid sequence of SEQ ID NO: 2, the mutations selected from groups (a) and (b) below:
(a) A1E/T, S9/T, P14L, A15L, L16C, H17T, S18L/T, Q19K, G20A, Y21N/T, T22L, G23E, S33T, K43Y, N76D, G102A, N109A/S/G, A137V, K141R, T158S, G169A, S204E, P210S, N218S, N243P/V, S248N/A, S249A, K256R, L257G, S260P, and N269D; and
(b) S24G, N25G, P40A/E, P52L, S53G, T55P, F58G, Q59S, N61E/P/G/S, N62Q/R/S, S63G/H, V68A, S78N, P86S, S87D/G, A88T/V, S89Y, A92G, L96T, G97A, G100N/Q/T, S101N, Q103E/H, Y104N, W106F, I111V, A114G, I115V, A116N/T, N117S, N118G, N123A/G/Q/V, M124I/V, S125A, L126A, G128A, P129E/Q/S/V, S130G, G131S/H, S132N, A133V, A134T, A144K, S145D, S159K, S161P, S162G/K, Y167A, P194L, V203Y, Q206D/E, K213L, Y217Q, V227T, A232T, P239R/V, N240K, T242R, K265N, L267V, and Q275E;
wherein the protease variant comprises the following mutations S24G, S33T, S53G, N76D, S78N, S101N, G128A, and Y217Q.