	US 12,338,267 B2
	Viral vectors exhibiting improved gene delivery properties
George M. Church, Brookline, MA (US); Eric Kelsic, Cambridge, MA (US); Pierce Ogden, Cambridge, MA (US); and Sam Sinai, Cambridge, MA (US)
Assigned to PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Appl. No. 17/055,318
Filed by PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
PCT Filed May 8, 2019, PCT No. PCT/US2019/031307 § 371(c)(1), (2) Date Nov. 13, 2020, PCT Pub. No. WO2019/221992, PCT Pub. Date Nov. 21, 2019.
Claims priority of provisional application 62/671,949, filed on May 15, 2018.
Prior Publication US 2021/0230229 A1, Jul. 29, 2021
Int. Cl. C07K 14/005 (2006.01); C12N 7/00 (2006.01); C12N 15/86 (2006.01)

CPC C07K 14/005 (2013.01) [C12N 7/00 (2013.01); C12N 15/86 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14145 (2013.01)]

16 Claims

1. An AAV2 capsid polypeptide comprising:

a mutation in the region of amino acids 561-588 of SEQ ID NO: 1 that increases tissue tropism of a virus to the kidney, wherein the region of amino acid 561-588 of SEQ ID NO: 1 has a sequence selected from the group consisting of: SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9;

a mutation in the region of amino acids 561-588 of SEQ ID NO: 1 that increases tissue tropism of a virus to the liver, wherein the region of amino acid 561-588 of SEQ ID NO: 1 has a sequence selected from the group consisting of: SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15; or

a mutation in the region of amino acids 561-588 of SEQ ID NO: 1 that increases tissue tropism of a virus to the lung, wherein the region of amino acid 561-588 of SEQ ID NO: 1 has a sequence selected from the group consisting of: SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, or SEQ ID NO: 20.