US 12,337,319 B2
Microfluidic chip for acoustic separation of biological objects
Bing K. Yen, Cupertino, CA (US); and Yuchen Zhou, San Jose, CA (US)
Assigned to Applied Cells Inc.
Filed by Applied Cells Inc., Santa Clara, CA (US)
Filed on Nov. 17, 2021, as Appl. No. 17/528,493.
Application 17/528,493 is a continuation in part of application No. 16/565,164, filed on Sep. 9, 2019, granted, now 11,541,391.
Application 16/565,164 is a continuation of application No. 15/911,116, filed on Mar. 3, 2018, granted, now 10,449,553, issued on Oct. 22, 2019.
Application 15/911,116 is a continuation of application No. 15/911,115, filed on Mar. 3, 2018, granted, now 12,145,151.
Prior Publication US 2022/0072548 A1, Mar. 10, 2022
Int. Cl. B01L 3/00 (2006.01); A61K 38/27 (2006.01); B01D 21/28 (2006.01); B01D 35/06 (2006.01); B01F 31/86 (2022.01); B03C 1/005 (2006.01); B03C 1/033 (2006.01); B03C 1/23 (2006.01); B03C 1/28 (2006.01); B03C 1/30 (2006.01); C12Q 1/6806 (2018.01); G01N 1/40 (2006.01); G01N 15/00 (2024.01); G01N 27/74 (2006.01); G01N 33/49 (2006.01); G01N 33/543 (2006.01); G01N 35/00 (2006.01)
CPC B01L 3/502753 (2013.01) [A61K 38/27 (2013.01); B01D 21/283 (2013.01); B01D 35/06 (2013.01); B01F 31/86 (2022.01); B01L 3/502715 (2013.01); B01L 3/50273 (2013.01); B01L 3/502761 (2013.01); B03C 1/005 (2013.01); B03C 1/0332 (2013.01); B03C 1/23 (2013.01); B03C 1/288 (2013.01); B03C 1/30 (2013.01); C12Q 1/6806 (2013.01); G01N 1/4077 (2013.01); G01N 15/00 (2013.01); G01N 27/745 (2013.01); G01N 33/491 (2013.01); G01N 33/54326 (2013.01); G01N 33/54333 (2013.01); G01N 35/0098 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0652 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/021 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0436 (2013.01); B01L 2400/0439 (2013.01); B01L 2400/0457 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0496 (2013.01); B03C 2201/18 (2013.01); B03C 2201/20 (2013.01); B03C 2201/26 (2013.01); G01N 2035/00554 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A microfluidic device for sorting biological objects comprising:
a microfluidic chip including:
a planar substrate having first and second planar surfaces, the planar substrate including first and second networks of channels recessed respectively from the first and second planar surfaces and directly connected by a through-hole in the planar substrate;
a first lid attached to the first planar surface of the planar substrate and covering the first network of channels; and
a second lid attached to the second planar surface of the planar substrate and covering the second network of channels; and
one or more first piezoelectric transducers attached to an exterior surface of the first lid, opposite to an interior surface of the first lid facing the first network of channels, and configured to generate a first acoustic standing wave having a first wavelength in a first linear channel of the first network of channels.