US 12,337,317 B2
Cell poration and transfection apparatuses
Alexander Govyadinov, Corvallis, OR (US); Viktor Shkolnikov, Palo Alto, CA (US); and Diane R. Hammerstad, Corvallis, OR (US)
Assigned to Hewlett-Packard Development Company, L.P., Spring, TX (US)
Appl. No. 17/416,565
Filed by Hewlett-Packard Development Company, L.P., Spring, TX (US)
PCT Filed Jul. 25, 2019, PCT No. PCT/US2019/043393
§ 371(c)(1), (2) Date Jun. 21, 2021,
PCT Pub. No. WO2021/015779, PCT Pub. Date Jan. 28, 2021.
Prior Publication US 2022/0184610 A1, Jun. 16, 2022
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/50273 (2013.01) [B01L 3/502715 (2013.01); B01L 2200/16 (2013.01); B01L 2300/1833 (2013.01); B01L 2400/0433 (2013.01); B01L 2400/0478 (2013.01)] 7 Claims
OG exemplary drawing
 
1. An apparatus, comprising:
a channel comprising an indentation to hold a cell at a first wall of the channel;
an energy source formed on a second wall of the channel to apply a shockwave to the cell in the channel to porate the cell, the energy source opposite the indentation at the first wall of the channel;
a transfection chamber at an opening formed by the first wall and the second wall of the channel and to store a reagent to be inserted into the cell after the cell is porated; and
a pump on a side of the channel opposite the transfection chamber, wherein the pump is to push the cell through the channel into the transfection chamber by pushing the cell into and out of the indentation and subsequently pushing the cell through the channel and through the opening into the transfection chamber.