US 12,337,316 B2
Plasma separating microfluidic device
Niclas Roxhed, Bromma (SE); Göran Stemme, Lidingö (SE); Gabriel Lenk, Freiburg (DE); Wouter Van Der Wijngaart, Sollentuna (SE); and Jonas Hansson, Stockholm (SE)
Assigned to CAPITAINER AB, Solna (SE)
Filed by CAPITAINER AB, Solna (SE)
Filed on May 10, 2021, as Appl. No. 17/315,399.
Application 17/315,399 is a division of application No. 15/847,958, filed on Dec. 20, 2017, granted, now 11,000,846.
Application 15/847,958 is a continuation of application No. PCT/SE2016/050591, filed on Jun. 17, 2016.
Claims priority of application No. 1500280-1 (SE), filed on Jun. 20, 2015.
Prior Publication US 2021/0260579 A1, Aug. 26, 2021
Int. Cl. B01L 3/00 (2006.01); G01N 1/40 (2006.01); G01N 33/49 (2006.01)
CPC B01L 3/50273 (2013.01) [B01L 3/502738 (2013.01); B01L 3/502753 (2013.01); G01N 1/4077 (2013.01); G01N 33/491 (2013.01); B01L 2200/025 (2013.01); B01L 2200/0605 (2013.01); B01L 2200/0684 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/126 (2013.01); B01L 2300/161 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0418 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0677 (2013.01); G01N 2001/4088 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A method of preparing and transporting a sample from whole blood comprising the steps of:
(i) arranging in a microfluidic device, a substantially flat porous membrane so that a lower surface of the porous membrane forms an intersection in an acute angle with a hydrophilic surface of a support structure, wherein said porous membrane extends diagonally between a channel bottom and a channel cover of a capillary channel configured to receive and accommodate a lateral capillary flow, wherein a portion of the lower surface of the porous membrane contacts a top of the channel cover;
(ii) applying at least 30 μl whole blood to an upper surface of the membrane and admitting separation of plasma through the membrane;
(iii) admitting a wedge-shaped space between the membrane and the support structure to be gradually filled with separated plasma; and
(iv) filling the capillary channel in fluid communication with said wedge-shaped space and metering a defined volume of separated plasma.