US 12,337,311 B2
Engineering novel enteroid models for understanding human enteric disease
Tatiana Y. Fofanova, Houston, TX (US); Jennifer Auchtung, Houston, TX (US); Reid Laurence Wilson, Houston, TX (US); Christopher Stewart, Houston, TX (US); Joseph Petrosino, Houston, TX (US); Robert Allen Britton, Houston, TX (US); Jane Grande-Allen, Houston, TX (US); Noah F. Shroyer, Houston, TX (US); and Mary K. Estes, Houston, TX (US)
Assigned to Baylor College of Medicine, Houston, TX (US); and William Marsh Rice University, Houston, TX (US)
Appl. No. 16/757,577
Filed by Baylor College of Medicine, Houston, TX (US); and William Marsh Rice University, Houston, TX (US)
PCT Filed Oct. 26, 2018, PCT No. PCT/US2018/057832
§ 371(c)(1), (2) Date Apr. 20, 2020,
PCT Pub. No. WO2019/084484, PCT Pub. Date May 2, 2019.
Claims priority of provisional application 62/577,221, filed on Oct. 26, 2017.
Prior Publication US 2020/0269230 A1, Aug. 27, 2020
Int. Cl. B01L 1/02 (2006.01); B01L 3/00 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12N 1/14 (2006.01); C12N 7/00 (2006.01); C40B 30/06 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01)
CPC B01L 1/025 (2013.01) [B01L 3/5085 (2013.01); C12M 25/02 (2013.01); C12M 25/04 (2013.01); C12M 41/34 (2013.01); C12N 1/14 (2013.01); C12N 7/00 (2013.01); C40B 30/06 (2013.01); G01N 33/5008 (2013.01); G01N 33/569 (2013.01); B01L 2300/042 (2013.01); B01L 2300/048 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A co-culture system for co-culturing anaerobic microbes and gut tissue, comprising two or more anaerobic chambers and two or more aerobic chambers, and a gas adjustable chamber comprising a gas;
wherein each anerobic chamber is removably insertable into a single aerobic chamber;
wherein each anaerobic chamber comprises a gas permeable base, wherein when a single anaerobic chamber is inserted into the single aerobic chamber, the single anaerobic chamber is gaseously communicable with the single aerobic chamber;
wherein the gas permeable base comprises gut tissue of a subject in the anaerobic chamber;
wherein the gut tissues comprises an apical side and a basolateral side; wherein the apical side faces the anaerobic chamber; wherein the apical side is anaerobic and the basolateral side is oxygenated;
and wherein there is a gas permeable side common to the two or more aerobic chambers and the gas adjustable chamber.