US 12,337,077 B2
Method for constructing bone morphogenetic protein slow-release system
Liyan Shen, Zhejiang (CN); Wenfei Ni, Zhejiang (CN); Yanlin Chen, Zhejiang (CN); and Chenqiang Jin, Zhejiang (CN)
Assigned to THE 2ND AFFILIATED HOSPITAL AND YUYING CHILDREN'S HOSPITAL OF WENZHOU MEDICAL UNIVERSITY, Zhejiang (CN)
Appl. No. 17/637,804
Filed by THE 2ND AFFILIATED HOSPITAL AND YUYING CHILDREN'S HOSPITAL OF WENZHOU MEDICAL UNIVERSITY, Zhejiang (CN)
PCT Filed Jul. 1, 2021, PCT No. PCT/CN2021/103960
§ 371(c)(1), (2) Date Feb. 24, 2022,
PCT Pub. No. WO2022/012339, PCT Pub. Date Jan. 20, 2022.
Claims priority of application No. 202010676616.6 (CN), filed on Jul. 14, 2020.
Prior Publication US 2022/0280687 A1, Sep. 8, 2022
Int. Cl. A61L 27/06 (2006.01); A61L 27/10 (2006.01); A61L 27/22 (2006.01); A61L 27/34 (2006.01); A61L 27/54 (2006.01)
CPC A61L 27/06 (2013.01) [A61L 27/10 (2013.01); A61L 27/227 (2013.01); A61L 27/34 (2013.01); A61L 27/54 (2013.01); A61L 2300/252 (2013.01); A61L 2300/412 (2013.01); A61L 2300/602 (2013.01); A61L 2420/02 (2013.01); A61L 2420/08 (2013.01); A61L 2430/02 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method for constructing a bone morphogenetic protein slow-release system, comprising the following steps:
I: activating a titanium material: washing and drying the titanium material, soaking the dried titanium material into a buffer solution containing dopamine, and reacting for 12-72 hours to obtain a polydopamine (PDA)-modified Ti base material;
II: activating ceramic powder: dissolving the ceramic powder and the dopamine into the buffer solution, vibrating and dispersing, reacting for 12-72 hours under stirring, and centrifuging to remove large particles, thereby obtaining a polydopamine-modified ceramic particles (PDA@HA) solution;
III: constructing a multilayer film: sequentially soaking the PDA-modified Ti base material in a positively-charged polyelectrolyte solution, a negatively-charged polyelectrolyte solution, a positively-charged polyelectrolyte solution and the polydopamine-modified ceramic particles solution for 8-15 min, and washing with water after soaking; and
IV: soaking in a circulating mode: soaking and cleaning in step III by circulating operation with circulating times of n times, thereby obtaining a positively-charged polyelectrolyte, negatively-charged polyelectrolyte and PDA-modified ceramic particles multilayer film.