	US 12,337,076 B2
	Application of nano layered double hydroxide in cartilage regeneration and preparation thereof
Rongrong Zhu, Shanghai (CN); and Liming Cheng, Shanghai (CN)
Assigned to Tongji University, Shanghai (CN)
Filed by Tongji University, Shanghai (CN)
Filed on Sep. 2, 2024, as Appl. No. 18/822,374.
Application 18/822,374 is a continuation of application No. PCT/CN2023/094575, filed on May 16, 2023.
Claims priority of application No. 202210528029.1 (CN), filed on May 16, 2022.
Prior Publication US 2024/0416002 A1, Dec. 19, 2024
Int. Cl. A61L 27/04 (2006.01); A61L 27/38 (2006.01)

CPC A61L 27/047 (2013.01) [A61L 27/3834 (2013.01); A61L 2400/12 (2013.01); A61L 2430/06 (2013.01)]

2 Claims

1. A method for preparing a nano layered double hydroxide (LDH), comprising:

step 1, preparing 60 milliliters (mL) salt solution by using 6 millimoles (mmol) magnesium nitrate and 2 mmol ferric nitrate, wherein a mole ratio of magnesium ion to ferric ion is 3:1;

step 2, preparing 40 ml of 0.016 moles per liter (M) sodium hydroxide solution by using double-distilled water with carbon dioxide removed as a solvent;

step 3, adding the 60 mL salt solution of step 1 to the 40 ml of 0.016 M sodium hydroxide solution of step 2 with stirring while continuously supplying nitrogen gas to obtain a first suspension;

step 4, transferring the first suspension to a hydrothermal synthesis reactor and then heating the first suspension at 100 degrees Celsius (C) for 18 hours to obtain a second suspension;

step 5, centrifuging the second suspension at 20000 gravitational accelerations (g) for 15 minutes (min) to obtain a product, washing the product twice with double-distilled water with carbon dioxide removed to obtain a gel-like material, and storing the gel-like material in a refrigerator at 4° C.; and

step 6, drying the gel-like material of step 5 in a vacuum drying oven to obtain the nano LDH; and

wherein the nano LDH is used to promote chondrogenic differentiation of human umbilical cord-derived mesenchymal stem cells.