US 12,014,511 B2
Sample imaging via two-pass light-field reconstruction
Raimund Leitner, Puch/Hallein (AT); and Jan Steinbrener, Puch/Hallein (AT)
Assigned to Molecular Devices (Austria) GmbH, (AT)
Appl. No. 17/634,491
Filed by MOLECULAR DEVICES (AUSTRIA) GMBH, Hallein (AT)
PCT Filed Aug. 11, 2020, PCT No. PCT/IB2020/057552
§ 371(c)(1), (2) Date Feb. 10, 2022,
PCT Pub. No. WO2021/028836, PCT Pub. Date Feb. 18, 2021.
Claims priority of provisional application 62/885,735, filed on Aug. 12, 2019.
Prior Publication US 2022/0270279 A1, Aug. 25, 2022
Int. Cl. G06T 7/557 (2017.01); G02B 21/36 (2006.01); G06T 3/10 (2024.01)
CPC G06T 7/557 (2017.01) [G02B 21/361 (2013.01); G02B 21/365 (2013.01); G06T 3/10 (2024.01); G06T 2207/10012 (2013.01); G06T 2207/10052 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of imaging a sample, the method comprising:
(a) capturing a light-field image of the sample in a light-field plane;
(b) forward-projecting the light-field image computationally to each of a plurality of z-planes in object space to generate a set of z-plane images;
(c) comparing, with respect to the light-field image, a backward projection to the light-field plane of the same xy-region in object space from each z-plane image, to determine a respective degree of correspondence between the backward-projected xy-region from each of the z-plane images and the light-field image, wherein comparing is repeated for each of a plurality of different xy-regions in object space;
(d) selecting based on comparing, for each different xy-region, at least one of the z-plane images to contribute data for the different xy-region in an object-space image of the sample; and
(e) generating the object-space image of the sample.