US 12,013,400 B2
Radical dosimetry methods for in vivo hydroxyl radical protein foot-printing
Scot Randy Weinberger, Montara, CA (US); Ronald Carl Orlando, Athens, GA (US); Joshua Shane Sharp, Oxford, MS (US); Robert Wallace Egan, Reno, NV (US); and Jeffrey Jonathan Persoff, San Jose, CA (US)
Assigned to GENNEXT TECHNOLOGIES, INC., Half Moon Bay, CA (US)
Filed by GenNext Technologies, Inc., Montara, CA (US)
Filed on Feb. 5, 2021, as Appl. No. 17/168,472.
Application 17/168,472 is a continuation in part of application No. PCT/US2020/012430, filed on Jan. 6, 2020.
Application PCT/US2020/012430 is a continuation in part of application No. 16/316,006, filed on Jan. 7, 2019, granted, now 10,816,468, issued on Oct. 27, 2020.
Application 17/168,472 is a continuation in part of application No. PCT/US2019/057059, filed on Oct. 18, 2019.
Application PCT/US2019/057059 is a continuation in part of application No. 16/316,006, granted, now 10,816,468, issued on Oct. 27, 2020, previously published as PCT/US2018/034682, filed on May 25, 2018.
Claims priority of provisional application 62/788,219, filed on Jan. 4, 2019.
Claims priority of provisional application 62/747,247, filed on Oct. 18, 2018.
Claims priority of provisional application 62/511,571, filed on May 26, 2017.
Claims priority of provisional application 63/128,439, filed on Dec. 21, 2020.
Prior Publication US 2021/0156869 A1, May 27, 2021
Int. Cl. G01N 33/68 (2006.01); C23C 18/14 (2006.01); G01N 21/59 (2006.01); G01N 21/63 (2006.01); G01N 21/64 (2006.01); H01J 49/00 (2006.01)
CPC G01N 33/6803 (2013.01) [C23C 18/14 (2013.01); G01N 21/59 (2013.01); G01N 21/631 (2013.01); G01N 21/6428 (2013.01); H01J 49/0036 (2013.01); G01N 2021/6439 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method of selective labeling of solvent exposed molecular groups, the method comprising:
introducing a sample mixture including a dosimeter internal standard into a photolysis cell of a flash photolysis system, wherein a change in dosimeter internal standard photometric absorbance represents an amount of produced free-radicals;
determining a photometric absorbance of the sample mixture;
irradiating the sample mixture with at least a first single pulse of photolysis light;
determining a photometric absorbance change of the irradiated sample mixture and adjusting a spectral irradiance of the photolysis light source in response to the determined change in photometric absorbance; and
analyzing products produced from the irradiated sample mixture, the analysis including detection of modified surface exposed functional groups.