| CPC G01N 33/56972 (2013.01) [B01L 3/50273 (2013.01); G01N 33/54366 (2013.01); G01N 33/569 (2013.01); C12Q 1/00 (2013.01); G01N 33/00 (2013.01)] | 15 Claims |
|
1. A microfluidic detection chip for the detection of infection in a patient comprising:
a. a plurality of microchannel layers in which are disposed a plurality of channels, wherein the plurality of channels comprise:
(i) a sample input channel into which a sample fluid can be inputted, wherein the sample fluid comprises one or more bodily fluids comprising active neutrophils and resting neutrophils;
(ii) one or more separation channels having one or more three-dimensional (3D) separation zones, wherein the one or more separation channels are operatively coupled to the sample input channel to receive the sample fluid and the one or more separation channels comprise an affinity surface and are operatively configured to serially capture, isolate, and deplete unwanted cells, and isolate the active neutrophils and resting neutrophils; and
(iii) one or more optical channels having one or more optical zones, wherein the one or more optical channels are operatively coupled to the one or more separation channels to receive the sample fluid and the one or more optical channels are operatively configured to enumerate the active neutrophils and resting neutrophils to provide detection of infection in the patient.
|