	US 12,013,391 B2
	Stabilized cell acquisition for elemental analysis
Vladimir Baranov, Richmond Hill (CA); Alexander Loboda, Thornhill (CA); and Michael Sullivan, Ontario (CA)
Assigned to STANDARD BIOTOOLS CANADA INC., Markham (CA)
Filed by Fluidigm Canada Inc., Markham (CA)
Filed on Apr. 12, 2019, as Appl. No. 16/383,241.
Claims priority of provisional application 62/657,332, filed on Apr. 13, 2018.
Prior Publication US 2019/0317082 A1, Oct. 17, 2019
Int. Cl. G01N 33/53 (2006.01); G01N 33/58 (2006.01); H01J 49/10 (2006.01)

CPC G01N 33/5306 (2013.01) [G01N 33/58 (2013.01); H01J 49/105 (2013.01); G01N 2458/15 (2013.01); G01N 2560/00 (2013.01)]

18 Claims

1. A sample-making kit, comprising element-tagged affinity reagent comprising an affinity reagent capable of specifically binding to an analyte and a metal-binding moiety bound to one or more metal elements; and

a stabilizing solution having a total dissolved solids at or below approximately 0.2%, wherein the stabilizing solution has a pH of between 5-9 and comprises a salt, wherein:

the salt is present in concentrations of 5 mM to 25 mM,

the salt is ammonium nitrate, and

the stabilizing solution prevents or reduces signal drop and deposition of the salt on an injector configured to convey a sample solution comprising the analyte, the element-tagged affinity reagent, and the stabilizing solution to an inductively coupled plasma.