US 12,012,630 B2
Quantitative acetaminophen analytics
Matthias Grimmler, Elz (DE); Detlef Thônges, Limburg (DE); Pia Schu, Schmelz (DE); Carolin Menzenbach, Pleckhausen (DE); and Laura Kreckel, Elz (DE)
Assigned to DiaSys Diagnostic Systems GmbH, Holzheim (DE)
Appl. No. 16/960,909
Filed by DiaSys Diagnostic Systems GmbH, Holzheim (DE)
PCT Filed Jan. 8, 2019, PCT No. PCT/EP2019/050291
§ 371(c)(1), (2) Date Jul. 9, 2020,
PCT Pub. No. WO2019/137890, PCT Pub. Date Jul. 18, 2019.
Claims priority of application No. 10 2018 100 426.5 (DE), filed on Jan. 10, 2018.
Prior Publication US 2020/0362386 A1, Nov. 19, 2020
Int. Cl. G01N 33/94 (2006.01); C12Q 1/34 (2006.01); G01N 33/52 (2006.01)
CPC C12Q 1/34 (2013.01) [G01N 33/52 (2013.01); G01N 33/9486 (2013.01); G01N 2333/98 (2013.01)] 10 Claims
 
1. A method for quantitatively determining an amount of acetaminophen in a sample, comprising:
producing a reaction mixture by hydrolyzing acetaminophen having formula (I)

OG Complex Work Unit Chemistry
resulting in p-aminophenol having formula (II)

OG Complex Work Unit Chemistry
and reacting the p-aminophenol having formula (II) with a compound of general formula (III)

OG Complex Work Unit Chemistry
in the presence of an oxidant to form a compound of general formula (IV)

OG Complex Work Unit Chemistry
wherein, in formulae (III) and (IV),
R1 and R2, independently of one another, are selected from H, —CH3, and —OCH3,
R3 is —C2H5 and
R4 is a substituted C1-4 alkyl moiety with a terminal sulfonate group,
with the proviso that at least one of R1 and R2 is —OCH3 and/or R4 additionally comprises at least one OH substituent,
and then an amount of the compound of general formula (IV) in the reaction mixture is photometrically determined.