US 12,012,620 B2
Peroxidase activity towards 10-acetyl-3,7-dihydroxyphenoxazine
Da Duan, Foster City, CA (US); Auric Anthony Sowell-Kantz, Mill Valley, CA (US); Aksiniya Lyubenova Petkova, Sunnyvale, CA (US); Jovana Nazor, Milpitas, CA (US); Nandhitha Subramanian, Cambridge (GB); and Oscar Alvizo, Fremont, CA (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Appl. No. 17/635,163
Filed by Codexis, Inc., Redwood City, CA (US)
PCT Filed Sep. 3, 2020, PCT No. PCT/US2020/049164
§ 371(c)(1), (2) Date Feb. 14, 2022,
PCT Pub. No. WO2021/050348, PCT Pub. Date Mar. 18, 2021.
Claims priority of provisional application 62/899,190, filed on Sep. 12, 2019.
Prior Publication US 2022/0290110 A1, Sep. 15, 2022
Int. Cl. C12N 9/08 (2006.01)
CPC C12N 9/0065 (2013.01) 12 Claims
 
1. An engineered peroxidase comprising a polypeptide sequence having, 90%, or more sequence identity to SEQ ID NO: 4, or a functional fragment thereof, wherein said engineered peroxidase comprises a substitution in said polypeptide sequence at position 393, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO: 4.