	US 12,012,617 B2
	Antibacterial and protective bacteriophage formulations and methods for making and using them
Forest Rohwer, San Diego, CA (US); and Jeremy J. Barr, San Diego, CA (US)
Assigned to San Diego State University (SDSU) Foundation, San Diego, CA (US)
Filed by San Diego State University (SDSU) Foundation, San Diego, CA (US)
Filed on Nov. 23, 2021, as Appl. No. 17/534,372.
Application 17/534,372 is a continuation of application No. 15/762,908, granted, now 11,214,773, previously published as PCT/US2016/052716, filed on Sep. 20, 2016.
Claims priority of provisional application 62/232,070, filed on Sep. 24, 2015.
Prior Publication US 2022/0162566 A1, May 26, 2022
Int. Cl. C12N 7/00 (2006.01); A61K 35/76 (2015.01); A61P 1/04 (2006.01); A61P 31/04 (2006.01); C07K 14/005 (2006.01)

CPC C12N 7/00 (2013.01) [A61K 35/76 (2013.01); A61P 31/04 (2018.01); C07K 14/005 (2013.01); C07K 2319/035 (2013.01); C12N 2795/00021 (2013.01); C12N 2795/00022 (2013.01); C12N 2795/00031 (2013.01); C12N 2795/00033 (2013.01); Y02A 50/30 (2018.01)]

28 Claims

1. A method for labelling or coating a cell with a payload or composition, or contacting a cell with a payload or composition, comprising:

contacting the cell with a genetically engineered bacteriophage of the genus Caudovirales,

wherein an exterior or outer surface of the bacteriophage comprises a payload or a composition, or the exterior or outer surface of the bacteriophage has attached thereto a payload or a composition, or a payload or a composition is adherent to the exterior or outer surface of the bacteriophage,

wherein the genetically engineered bacteriophage genome is altered such that after reproduction in a host cell, or in an in vitro system, the exterior or outer surface of the genetically engineered bacteriophage expresses or displays:

(a) on a tailspike protein at least one non-bacteriophage carbohydrate binding domain (CBD) comprising a lectin;

(b) at least one heterologous bacteriophage Ig-like CBD; or