	US 12,012,608 B2
	Transgenic maize plant exhibiting increased yield and drought tolerance
Karl Pioch, Gottingen (DE); Wolfgang Koch, Einbeck (DE); and Stefan Meldau, Gottingen (DE)
Assigned to KWS SAAT SE & Co. KGaA, Einbeck (DE)
Filed by KWS SAAT SE & Co. KGaA, Einbeck (DE)
Filed on Oct. 17, 2022, as Appl. No. 18/046,988.
Application 18/046,988 is a continuation of application No. 16/481,727, granted, now 11,505,802, previously published as PCT/EP2018/052315, filed on Jan. 30, 2018.
Claims priority of application No. 17153839 (EP), filed on Jan. 30, 2017.
Prior Publication US 2023/0250443 A1, Aug. 10, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); C12N 9/44 (2006.01); C12P 5/02 (2006.01); C12P 7/10 (2006.01)

CPC C12N 15/8261 (2013.01) [C12N 9/2451 (2013.01); C12N 15/8273 (2013.01); C12P 5/023 (2013.01); C12P 7/10 (2013.01); C12Y 302/01026 (2013.01)]

8 Claims

1. A transgenic maize plant comprising as transgene stably integrated into the genome of the maize plant:

i) an expression cassette comprising a nucleic acid capable of expressing a Chenopodium rubrum cell wall invertase according to SEQ ID NO: 3,

wherein the nucleic acid is constitutively overexpressed more than 10-fold relative to endogenous control gene ZmEF1, and

wherein as a result of the constitutive overexpression of the cell wall invertase, the transgenic maize plant exhibits an enhanced tolerance to drought and an increased biomass yield, optionally as compared to a reference.