	US 12,012,605 B2
	Generating northern leaf blight resistant maize
Huirong Gao, Johnston, IA (US); Bailin Li, Johnston, IA (US); Robert B Meeley, Des Moines, IA (US); Leandro Daniel Perugini, Urbandale, IA (US); and Girma M Tabor, Johnston, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., , IA (US)
Appl. No. 16/341,531
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
PCT Filed Oct. 10, 2017, PCT No. PCT/US2017/055835 § 371(c)(1), (2) Date Apr. 12, 2019, PCT Pub. No. WO2018/071362, PCT Pub. Date Apr. 19, 2018.
Claims priority of provisional application 62/407,867, filed on Oct. 13, 2016.
Prior Publication US 2022/0275392 A1, Sep. 1, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/82 (2013.01) [C12N 15/8213 (2013.01); C12N 15/8282 (2013.01)]

18 Claims

1. A method for obtaining a maize plant cell with a modified Ht1 nucleotide sequence, the method comprising:

a) introducing a site-specific modification at at least one target site in an endogenous genomic locus encoding Ht1 in a plant cell;

b) obtaining the maize plant cell having a modified Ht1 nucleotide sequence; and

c) introducing an Ht1 substitution template in the maize plant cell, wherein said Ht1 substitution template comprises at least one nucleic acid alteration compared to the endogenous Ht1 encoding sequence, allele, or genomic locus and wherein said Ht1 substitution template is incorporated into the endogenous genomic locus encoding Ht1,

wherein said Ht1 substitution template comprises SEQ ID NO:59, SEQ ID NO: 65, or a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:52.