	US 12,012,604 B2
	Acetate consuming yeast cell
Paul Klaassen, Echt (NL); Jozef Petrus Johannes Schmitz, Echt (NL); Paulus Petrus De Waal, Echt (NL); and Arjen Bouma, Echt (NL)
Assigned to Danisco US Inc., Palo Alto, CA (US)
Filed by DANISCO US INC., Palo Alto, CA (US)
Filed on Jun. 14, 2021, as Appl. No. 17/347,204.
Application 17/347,204 is a continuation of application No. 15/575,538, granted, now 11,319,543, previously published as PCT/EP2016/061099, filed on May 18, 2016.
Claims priority of application No. 15168833 (EP), filed on May 22, 2015.
Prior Publication US 2021/0310013 A1, Oct. 7, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/81 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/12 (2006.01); C12N 9/16 (2006.01); C12N 9/92 (2006.01); C12P 7/06 (2006.01); C12P 7/10 (2006.01)

CPC C12N 15/81 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/1205 (2013.01); C12N 9/16 (2013.01); C12N 9/92 (2013.01); C12N 9/93 (2013.01); C12P 7/06 (2013.01); C12P 7/10 (2013.01); C12Y 102/0101 (2013.01); C12Y 207/01028 (2013.01); C12Y 207/01029 (2013.01); C12Y 301/03021 (2013.01); C12Y 503/01005 (2013.01); C12Y 602/01001 (2013.01); C12P 2203/00 (2013.01); Y02E 50/10 (2013.01)]

14 Claims

1. A yeast cell that is genetically modified comprising:

a) a disruption of one or more aldehyde dehydrogenase (E.C:1.2.1.4) native to the yeast;

b) one or more nucleotide sequence encoding a heterologous NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10);

c) one or more nucleotide sequence encoding a homologous or heterologous acetyl-CoA synthetase (E.C. 6.2.1.1); and

d) a modification that leads to reduction of glycerol 3-phosphate phosphohydrolase (E.C. 3.1.3.21) and/or glycerol 3-phosphate dehydrogenase (E.C. 1.1.1.8 or E.C. 1.1.5.3) activity, native to the yeast.