	US 12,012,603 B2
	Yeast strains with selected or altered mitotypes and methods of making and using the same
Chris Todd Hittinger, Madison, WI (US); David Peris Navarro, Valencia (ES); and EmilyClare Patricia Baker, Madison, WI (US)
Assigned to WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (US)
Filed by Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed on Aug. 7, 2019, as Appl. No. 16/533,928.
Claims priority of provisional application 62/715,498, filed on Aug. 7, 2018.
Prior Publication US 2020/0048645 A1, Feb. 13, 2020
Int. Cl. C12N 15/81 (2006.01); C12N 1/14 (2006.01); C12N 1/18 (2006.01); C12R 1/645 (2006.01)

CPC C12N 15/81 (2013.01) [C12N 1/145 (2021.05); C12N 1/18 (2013.01); C12R 2001/645 (2021.05)]

7 Claims

1. A method of making a hybrid yeast strain having a selected mitotype comprising:

treating a first yeast strain with a mitochondria elimination agent to produce a first mitochondria-null yeast strain, and

mating the first mitochondria-null yeast strain with a second yeast strain comprising mitochondria to produce the yeast strain having the mitotype of the second yeast strain,

wherein the mitotype of the second yeast strain confers increased cold tolerance relative to the first yeast strain.