US 12,012,584 B2
Cell culture plate, devices and methods for in vitro exposure
David Bovard, Zug (CH); Julia Hoeng, Corcelles (CH); Shoaib Majeed, Neuchatel (CH); and Antonin Sandoz, Geneva (CH)
Assigned to Philip Morris Products S.A., Neuchatel (CH)
Appl. No. 16/640,401
Filed by PHILIP MORRIS PRODUCTS S.A., Neuchatel (CH)
PCT Filed Aug. 30, 2018, PCT No. PCT/EP2018/073409
§ 371(c)(1), (2) Date Feb. 20, 2020,
PCT Pub. No. WO2019/043130, PCT Pub. Date Mar. 7, 2019.
Claims priority of application No. 17188871 (EP), filed on Aug. 31, 2017; and application No. 17208969 (EP), filed on Dec. 20, 2017.
Prior Publication US 2020/0339938 A1, Oct. 29, 2020
Int. Cl. C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/32 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12M 1/42 (2006.01)
CPC C12M 41/14 (2013.01) [C12M 23/12 (2013.01); C12M 23/20 (2013.01); C12M 23/26 (2013.01); C12M 23/38 (2013.01); C12M 23/40 (2013.01); C12M 25/04 (2013.01); C12M 29/12 (2013.01); C12M 35/08 (2013.01); C12M 41/46 (2013.01); C12M 41/48 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A cell culture plate comprising:
at least two sequentially arranged wells; and
a singular, unitary, and continuous channel having (i) a wall extending from a first end to a second end, (ii) a first end opening at the first end, (iii) a second end opening at the second end, (iv) a first well communication opening in the wall between the first end and the second end, and (v) a second well communication opening in the wall between the first end and the second end,
wherein the first end of the channel is connected or coupled to a first pump via the first end opening,
wherein the second end of the channel is connected or coupled to the first pump via the second end opening,
wherein a first of the at least two sequentially arranged wells is in communication with the channel via the first well communication opening,
wherein a second of the at least two sequentially arranged wells is in communication with the channel via the second well communication opening, and
wherein the first pump is operable to circulate fluid between the at least two wells.