US 12,012,488 B2
Dual-network collagen-based supramolecular hydrogel and its preparation method
Jiabo Shi, Xi'an (CN); Yuxuan Zhang, Xi'an (CN); Jikai Yang, Xi'an (CN); Ruizhen Zhang, Xi'an (CN); Yuxin Du, Xi'an (CN); and Xinchun Chen, Xi'an (CN)
Assigned to Shaanxi University of Science & Technology, Xi'an (CN)
Filed by Shaanxi University of Science & Technology, Xi'an (CN)
Filed on Jun. 27, 2023, as Appl. No. 18/341,874.
Claims priority of application No. 202210748429.3 (CN), filed on Jun. 29, 2022.
Prior Publication US 2023/0331938 A1, Oct. 19, 2023
Int. Cl. C08J 3/075 (2006.01); C08K 3/36 (2006.01)
CPC C08J 3/075 (2013.01) [C08K 3/36 (2013.01); C08J 2389/06 (2013.01); C08K 2201/011 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method for preparing a dual-network collagen-based supramolecular hydrogel, comprising:
(1) dispersing a silicate nanosheet in deionized water under magnetic stirring for 2 h to obtain a silicate nanosheet dispersion; dissolving a lyophilized type I collagen sponge with a phosphate buffered solution (PBS) at 4° C. in an ice bath to obtain a collagen solution with a concentration of 10 mg/mL; and adding the nanosheet dispersion to the collagen solution followed by stirring in an ice bath for 2 h to obtain a collagen-silicate nanosheet network as network A, wherein a weight ratio of the lyophilized type I collagen sponge to the silicate nanosheet is 1:0.01˜0.2;
(2) dissolving tannic acid in deionized water under ultrasonication to obtain a tannic acid solution with a concentration of 10˜40 mg/mL; adding polyethylene glycol into deionized water followed by magnetic stirring at 35° C. to obtain a polyethylene glycol solution with a concentration of 20 mg/mL; and adding the tannic acid solution to the polyethylene glycol solution in a volume ratio of 1:1 followed by stirring for 5 h to obtain a polyethylene glycol-tannic acid network as network B; and
(3) adding the network B obtained in step (2) into the network A obtained in step (1) at 25° C. followed by magnetic stirring for 2 h to obtain a reaction mixture; and adjusting pH of the reaction mixture to weak acidity followed by standing for 12 h for complete reaction to obtain the dual-network collagen-based supramolecular hydrogel.