US 12,011,713 B2
System and method for sequestered wash buffer reuse
Wesley A. Cox-Muranami, San Diego, CA (US); Kay Klausing, San Diego, CA (US); Bradley Kent Drews, Poway, CA (US); Nicholas Watson, San Diego, CA (US); Jennifer Olivia Foley, San Diego, CA (US); Murphy Hitchcock, San Diego, CA (US); Paul Sangiorgio, San Diego, CA (US); and Sz-Chin Steven Lin, San Diego, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on Aug. 3, 2022, as Appl. No. 17/817,204.
Application 17/817,204 is a division of application No. 16/584,353, filed on Sep. 26, 2019, granted, now 11,426,723.
Claims priority of provisional application 62/741,812, filed on Oct. 5, 2018.
Prior Publication US 2022/0371005 A1, Nov. 24, 2022
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502 (2013.01) [B01L 2200/141 (2013.01); B01L 2200/16 (2013.01); B01L 2300/047 (2013.01); B01L 2300/06 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0644 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A system comprising:
a fluidic device;
a first reagent fluid reservoir fluidly connectable to the fluidic device;
a first fluid buffer reservoir fluidly connectable to the fluidic device;
a common fluid buffer source fluidly connectable to the fluidic device;
a pump;
a flow control valve for selectively connecting one of the first reagent fluid reservoir, the first fluid buffer reservoir, and the common fluid buffer source to the fluidic device; and
a controller in operative communication with the pump and the flow control valve and programmed to control the pump and the flow control valve to sequentially:
(i) effect fluid flow from the first reagent fluid reservoir to the fluidic device,
(ii) effect fluid flow from the common fluid buffer source to the fluidic device,
(iii) effect fluid flow from the fluidic device to the first fluid buffer reservoir,
(iv) effect fluid flow from the first reagent fluid reservoir to the fluidic device, and
(v) effect fluid flow from the first fluid buffer reservoir to the fluidic device.