US 12,011,694 B2
Crosslinked protein-based separation membrane and application thereof
Peng Yang, Shaanxi (CN); and Facui Yang, Shaanxi (CN)
Assigned to SHAANXI NORMAL UNIVERSITY, Shaanxi (CN)
Filed by SHAANXI NORMAL UNIVERSITY, Shaanxi (CN)
Filed on Jan. 19, 2022, as Appl. No. 17/579,209.
Application 17/579,209 is a continuation of application No. 17/263,264, abandoned, previously published as PCT/CN2019/085848, filed on May 7, 2019.
Claims priority of application No. 201810456238.3 (CN), filed on May 14, 2018.
Prior Publication US 2022/0134294 A1, May 5, 2022
Int. Cl. B01D 71/44 (2006.01); B01D 61/24 (2006.01); B01D 67/00 (2006.01); B01D 69/10 (2006.01); B01D 69/12 (2006.01); B01D 71/48 (2006.01)
CPC B01D 71/441 (2022.08) [B01D 61/243 (2013.01); B01D 67/0006 (2013.01); B01D 67/0093 (2013.01); B01D 69/1071 (2022.08); B01D 69/1251 (2022.08); B01D 71/48 (2013.01); B01D 2325/0283 (2022.08); B01D 2325/04 (2013.01)] 1 Claim
OG exemplary drawing
 
1. A method for dialysis using a crosslinked lysozyme-based dialysis membrane,
wherein the crosslinked lysozyme-based dialysis membrane comprising
a glutaraldehyde crosslinked lysozyme nanomembrane as a dense surface layer, and
a PET nuclear track membrane as a support layer,
the glutaraldehyde crosslinked lysozyme nanomembrane is attached on the PET nuclear track membrane, and
the glutaraldehyde crosslinked lysozyme nanomembrane is formed by crosslinking a lysozyme two-dimensional nanomembrane with glutaraldehyde, and the lysozyme two-dimensional nanomembrane is formed by lysozyme phase transition;
wherein the lysozyme two-dimensional nanomembrane is formed by a method comprising:
adjusting a pH value of a 10-100 mmol/L 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid buffered solution of tris(2-carboxyethyl) phosphine to 6.0-8.0 by using NaOH to get an adjusted solution, then mixing the adjusted solution with a 1-30 mg/mL 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid buffered solution of lysozyme in an isovolumetric manner to get a mixed solution, spreading the mixed solution to a whole surface of a substrate directly, incubating at room temperature for 2-6 hours, forming a layer of the lysozyme two-dimensional nanomembrane on a gas-liquid interface of a liquid on the substrate;
the glutaraldehyde crosslinked lysozyme nanomembrane is formed by a method comprising:
transferring the lysozyme two-dimensional nanomembrane to a glutaraldehyde aqueous solution with a mass fraction of 0.2%-2% for crosslinking for 2-6 hours at the room temperature;
wherein a thickness of the PET nuclear track membrane is 12 μm, and a pore size of the PET nuclear track membrane is in a range of 1-10 μm; and
wherein the dialysis comprises separating of mixed proteins from each other, the mixed proteins are bovine serum albumin and insulin, or muscle hemoglobin and insulin;
the dialysis comprises separating of mixed dyes from each other, the mixed dyes are methyl blue and methyl orange, or methyl blue and Rhodamine B; or
the dialysis comprises removing urotoxin from blood, the urotoxin is any one of urea, creatinine, β-microglobulin and indoxyl sulfate.