	US 12,011,505 B2
	Nanovesicles derived from cell membrane, and use thereof
Yong Song Gho, Pohang-si (KR); Dong-Sic Choi, Pohang-si (KR); and Gyeongyun Go, Pohang-si (KR)
Assigned to SL BIGEN, INC., Incheon (KR)
Filed by POSTECH ACADEMY-INDUSTRY FOUNDATION, Pohang-si (KR)
Filed on Mar. 25, 2021, as Appl. No. 17/212,131.
Application 17/212,131 is a division of application No. 15/545,398, granted, now 10,973,765, previously published as PCT/KR2015/008801, filed on Aug. 24, 2015.
Claims priority of application No. 10-2015-0014164 (KR), filed on Jan. 29, 2015.
Prior Publication US 2021/0212948 A1, Jul. 15, 2021
Int. Cl. A61K 9/127 (2006.01); A61K 9/50 (2006.01); A61K 9/51 (2006.01); A61K 31/704 (2006.01); A61K 31/713 (2006.01); A61K 33/26 (2006.01); A61K 38/46 (2006.01); A61K 47/50 (2017.01); A61K 48/00 (2006.01); A61K 49/00 (2006.01)

CPC A61K 9/1273 (2013.01) [A61K 9/5068 (2013.01); A61K 9/5089 (2013.01); A61K 9/5192 (2013.01); A61K 31/704 (2013.01); A61K 31/713 (2013.01); A61K 33/26 (2013.01); A61K 38/465 (2013.01); A61K 47/50 (2017.08); A61K 48/00 (2013.01); A61K 49/00 (2013.01); A61K 49/0067 (2013.01); A61K 49/0086 (2013.01); C12Y 301/27005 (2013.01)]

3 Claims

1. A method of delivering substances for treating or diagnosing diseases, the method comprising a step of delivering nanovesicles prepared from membrane of cells,

wherein the nanovesicles are loaded with one or more substances for treating or diagnosing a disease and the nanovesicles are targeted into cells or tissues,

wherein intracellular components including genetic materials and cytosolic proteins are removed in the nanovesicles by treating the cells with an alkaline solution of pH 9 to 14,

wherein the nanovesicles are loaded with the one or more substances for treating or diagnosing a disease selected from the group consisting of an anti-cancer agent, an anti-inflammatory agent, an angiogenesis inhibitor, a peptide, a protein, a toxin, a nucleic acid molecule, a nanoparticle, and a fluorescence-emitting material.