	US 12,011,464 B2
	Composition comprising induced exosome for hair regeneration
Yong Seung Lee, Suwon-si (KR)
Assigned to STEMON INC., Seongnam-si (KR)
Appl. No. 17/276,230
Filed by STEMON INC., Seongnam-si (KR)
PCT Filed Sep. 19, 2019, PCT No. PCT/KR2019/012134 § 371(c)(1), (2) Date Mar. 15, 2021, PCT Pub. No. WO2020/071662, PCT Pub. Date Apr. 9, 2020.
Claims priority of application No. 10-2018-0117263 (KR), filed on Oct. 2, 2018.
Prior Publication US 2022/0040238 A1, Feb. 10, 2022
Int. Cl. A61K 35/36 (2015.01); A61P 17/14 (2006.01); C12N 5/071 (2010.01); C12N 13/00 (2006.01)

CPC A61K 35/36 (2013.01) [A61P 17/14 (2018.01); C12N 5/0625 (2013.01); C12N 13/00 (2013.01); C12N 2529/00 (2013.01)]

8 Claims

1. A method for producing exosomes having a hair regeneration effect in a human, the method comprising:

a) providing ultrasound stimulation directly and indirectly to stem cells;

b) culturing a mixture of the stem cells and a medium for a predetermined time to provide a supernatant; and

c) isolating exosomes from the supernatant by storing the supernatant at 4° C. or below for 7 days to 1 month before filtering the supernatant through a filter;

wherein the providing the ultrasound stimulation directly to the stem cells is applying ultrasound stimulation to a medium containing the stem cells and the providing the stimulation indirectly to the stem cells is applying ultrasound stimulation to a medium not containing the stem cells.