US 12,011,461 B2
Compositions and methods of hematopoietic stem cell transplants
Ivan K. Dimov, Menlo Park, CA (US); Nathaniel Fernhoff, Menlo Park, CA (US); and Kevin Sheehan, Menlo Park, CA (US)
Assigned to Orca Biosystems, Inc., Menlo Park, CA (US)
Filed by Orca Biosystems, Inc., Menlo Park, CA (US)
Filed on Nov. 6, 2020, as Appl. No. 17/091,363.
Application 17/091,363 is a division of application No. 16/388,095, filed on Apr. 18, 2019, granted, now 10,857,183.
Application 16/388,095 is a continuation of application No. 15/922,709, filed on Mar. 15, 2018, granted, now 10,300,090, issued on May 28, 2019.
Claims priority of provisional application 62/471,769, filed on Mar. 15, 2017.
Prior Publication US 2021/0244763 A1, Aug. 12, 2021
Int. Cl. A61K 35/17 (2015.01); A61P 3/00 (2006.01); A61P 7/06 (2006.01); A61P 21/00 (2006.01); A61P 35/02 (2006.01); A61P 37/02 (2006.01); C12N 5/00 (2006.01); C12N 5/0783 (2010.01); C12N 5/0789 (2010.01)
CPC A61K 35/17 (2013.01) [A61P 3/00 (2018.01); A61P 7/06 (2018.01); A61P 21/00 (2018.01); A61P 35/02 (2018.01); A61P 37/02 (2018.01); C12N 5/0087 (2013.01); C12N 5/0636 (2013.01); C12N 5/0637 (2013.01); C12N 5/0638 (2013.01); C12N 5/0646 (2013.01); C12N 5/0647 (2013.01)] 21 Claims
 
1. A therapeutic composition comprising one or more unit doses of a cellular graft, wherein each unit dose of the cellular graft comprises a population of cells that is enriched for:
CD25+ regulatory T cells (Tregs); and
CD45RA− memory T cells (Tmems);
wherein the population of cells is depleted of CD45RA+CD25− naïve conventional αβ-T cells; wherein the population of cells comprises a ratio of CD45RA+CD25− naïve conventional αβ-T cells to CD25+ Tregs that is less than 1:5; wherein said therapeutic composition further comprises one or more pharmaceutically acceptable excipients; and wherein said therapeutic composition has a reduced incidence of Graft versus Host Disease (GVHD) following administration to a subject compared to administration of a composition comprising the ratio of CD45RA+CD25− naïve conventional αβ-T cells to CD25+ Tregs present in an unprocessed biological sample.