CPC A01N 1/0247 (2013.01) [A01N 1/02 (2013.01); A01N 1/0273 (2013.01)] | 22 Claims |
1. A method comprising:
receiving an ex vivo heart in a protective chamber of an organ care system;
pumping a perfusion fluid, via a perfusion circuit coupled to the ex vivo heart, into the ex vivo heart;
measuring an aortic pressure of the ex vivo heart;
obtaining a plurality of lactate differentials over time, wherein obtaining each lactate differential of the plurality of lactate differentials over time comprises:
measuring a first lactate value of the perfusion fluid in a first conduit, wherein the perfusion fluid in the first conduit is flowing into the ex vivo heart;
measuring a second lactate value of the perfusion fluid in a second conduit, wherein the perfusion fluid in the second conduit is flowing away from the ex vivo heart; and
subtracting the first lactate value of the perfusion fluid in the first conduit from the second lactate value of the perfusion fluid in the second conduit to obtain a lactate differential; and
correlating the plurality of lactate differentials over time and the aortic pressure with a transplantation suitability of the ex vivo heart, wherein at least two values of zero or less of the plurality of lactate differentials over time indicates that the ex vivo heart is suitable for transplantation.
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