US 12,332,417 B2
Optical system for a light sheet microscope
Christian Schumann, Lich (DE)
Assigned to LEICA MICROSYSTEMS CMS GMBH, Wetzlar (DE)
Appl. No. 17/770,321
Filed by LEICA MICROSYSTEMS CMS GMBH, Wetzlar (DE)
PCT Filed Oct. 22, 2020, PCT No. PCT/EP2020/079681
§ 371(c)(1), (2) Date Apr. 20, 2022,
PCT Pub. No. WO2021/078825, PCT Pub. Date Apr. 29, 2021.
Claims priority of application No. 10 2019 128 681.6 (DE), filed on Oct. 23, 2019.
Prior Publication US 2022/0390730 A1, Dec. 8, 2022
Int. Cl. G02B 21/00 (2006.01); G02B 21/36 (2006.01)
CPC G02B 21/0032 (2013.01) [G02B 21/367 (2013.01)] 20 Claims
OG exemplary drawing
 
1. An optical system for a light-sheet microscope, the optical system comprising:
transporting optics configured to project, into a sample, a light sheet for illuminating a sample plane positioned obliquely to an optical axis of the transporting optics and to project the illuminated sample plane into an intermediate image space,
wherein the transporting optics comprises an interchanging system that includes a first light-deflection element and a second light-deflection element, the interchanging system being configured to switch an illumination direction along which the light sheet illuminates the sample by alternately introducing the first light-deflection element and the second light-deflection element into a beam path of the transporting optics, so that the light sheet illuminates the sample from a first direction when only the first light-deflection element is in the beam path, and the light sheet illuminates the sample from a second direction when only the second light-deflection element is in the beam path, the second direction being different from the first direction, and
wherein the first light-deflection element causes a partial image inversion in only one direction and the second light-deflection element causes a complete image inversion in two directions.