	US 12,332,249 B1
	Biomolecule detection method using diffusion ordered spectroscopy
Abdulrahman Miteb Alazemi, Safat (KW); Fouzi Mouffouk, Safat (KW); Laila Abdulmohsen Alhaddad, Safat (KW); Abdulhamid Mohammed Emwas, Thuwal (SA); Mohamed Khaled Emira, Safat (KW); and Tasneem Salem Habib, Safat (KW)
Assigned to KUWAIT UNIVERSITY, Safat (KW)
Filed by KUWAIT UNIVERSITY, Safat (KW)
Filed on Oct. 22, 2024, as Appl. No. 18/923,470.
Int. Cl. G01N 33/532 (2006.01); G01N 33/558 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01)

CPC G01N 33/6845 (2013.01) [G01N 33/582 (2013.01)]

6 Claims

1. A method for detecting a presence of a second target biomolecule in a sample, the method comprising:

preparing a molecular tracer;

binding the molecular tracer to a first target biomolecule to provide a tracer bound-first target biomolecule;

contacting the tracer bound-first target biomolecule with the sample;

determining a first diffusion coefficient value for the tracer bound-first target biomolecule before contacting the tracer bound first-target biomolecule with the sample;

determining a second diffusion coefficient value for the tracer bound-first target biomolecule after contacting the tracer bound-first target biomolecule with the sample; and

determining difference in diffusion coefficient values between the first and the second diffusion coefficient values, wherein detecting a change of four orders of magnitude in the diffusion coefficient values indicates binding of the second target biomolecule with the tracer bound-first target biomolecule, thereby detecting the presence of the second target biomolecule in the sample;

wherein the molecular tracer comprises pentadecafluorooctanoyl; and wherein the first target biomolecule is biotin; and wherein the second target biomolecule is avidin.