US 12,332,248 B2
Sequence-detection system
Jeffrey G. Mandell, San Diego, CA (US); Kevin L. Gunderson, San Diego, CA (US); Michael Gregory Keehan, San Diego, CA (US); Erin Christine Garcia, San Diego, CA (US); and Jens H. Gundlach, San Diego, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Appl. No. 16/623,562
Filed by Illumina, Inc., San Diego, CA (US)
PCT Filed Sep. 14, 2018, PCT No. PCT/US2018/051212
§ 371(c)(1), (2) Date Dec. 17, 2019,
PCT Pub. No. WO2019/055867, PCT Pub. Date Mar. 21, 2019.
Claims priority of provisional application 62/559,202, filed on Sep. 15, 2017.
Prior Publication US 2024/0003896 A1, Jan. 4, 2024
Int. Cl. G01N 33/68 (2006.01); G16B 40/10 (2019.01)
CPC G01N 33/6818 (2013.01) [G16B 40/10 (2019.02)] 18 Claims
 
1. A sequence-detection system comprising:
a mechanical-change component that exhibits one or more mechanical changes when specifically interacting with entities within a target, each entity having a type;
a mechanical-change-to-signal transducer that transduces the one or more mechanical changes into an electrical signal;
a mechanical coupler that couples the mechanical-change component to the mechanical-change-to-signal transducer; and
an analysis subsystem that determines the types of entities within the target using the electrical signal by mapping two or more values derived from the electrical signal to a range volume, corresponding to the entity type, that does not overlap a range volume corresponding to any other entity type;
wherein the target is a primer-associated DNA template polymer;
wherein the mechanical-change component is a DNA polymerase;
wherein the mechanical coupler is a DNA-polymer tether attached, at one end, to the DNA polymerase; and
wherein the mechanical-change-to-signal transducer further comprises a variable resistor: (i) that regulates flow of an electrical current, and (ii) is a reporter-region of the DNA-polymer tether that lies within a constriction within a porin channel.