	US 12,332,241 B2
	Apoptotic cell mimic
Keunho Ahn, Pleasanton, CA (US); Zhenyu Deng, Berkeley, CA (US); Martina De Geus, Berkeley, CA (US); and Anh Tuan Nguyen, San Francisco, CA (US)
Assigned to Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed by Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed on Sep. 16, 2024, as Appl. No. 18/886,396.
Application 18/886,396 is a continuation of application No. 18/608,025, filed on Mar. 18, 2024, granted, now 12,130,285.
Application 18/608,025 is a continuation of application No. PCT/US2023/067893, filed on Jun. 2, 2023.
Claims priority of provisional application 63/348,414, filed on Jun. 2, 2022.
Prior Publication US 2025/0003960 A1, Jan. 2, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/543 (2006.01); G01N 15/10 (2024.01); G01N 15/14 (2024.01)

CPC G01N 33/5436 (2013.01) [G01N 15/1012 (2013.01); G01N 15/14 (2013.01); G01N 2015/1006 (2013.01)]

25 Claims

1. A kit comprising:

a) a first population of hydrogel beads, each hydrogel bead comprising:

i) a polymerized monomer;

ii) annexin V; and

iii) double stranded DNA;

b) a second population of hydrogel beads, each hydrogel bead comprising:

i) a polymerized monomer;

ii) annexin V; but

iii) lacking the double stranded DNA of the first population of hydrogel beads;

c) a third population of hydrogel beads, each hydrogel population comprising:

i) a polymerized monomer; but

ii) lacking the annexin V of the first population of hydrogel beads and the second population of hydrogel beads; and

iii) lacking the double stranded DNA of the first population of hydrogel beads;

wherein the annexin V of the first and/or second population of hydrogel beads comprises SEQ ID NO: 102.