US 12,332,238 B2
Extraction and detection of pathogens using carbohydrate-functionalized biosensors
Evangelyn C. Alocilja, East Lansing, MI (US); Barbara Christine Cloutier, Dewitt, MI (US); and Michael J. Anderson, Manassas, VA (US)
Assigned to BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY, East Lansing, MI (US); and USA AS REPRESENTED BY THE SECRETARY OF THE ARMY ON BEHALF OF USAMRMC, Fort Detrick, MD (US)
Filed by BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY, East Lansing, MI (US); and USA AS REPRESENTED BY THE SECRETARY OF THE ARMY ON BEHALF OF USAMRMC, Fort Detrick, MD (US)
Filed on Aug. 11, 2020, as Appl. No. 16/990,056.
Application 16/990,056 is a division of application No. 13/598,160, filed on Aug. 29, 2012, granted, now 10,739,337.
Claims priority of provisional application 61/528,892, filed on Aug. 30, 2011.
Prior Publication US 2020/0371094 A1, Nov. 26, 2020
Int. Cl. G01N 33/543 (2006.01); G01N 27/327 (2006.01); G01N 33/569 (2006.01)
CPC G01N 33/5434 (2013.01) [G01N 27/327 (2013.01); G01N 33/54393 (2013.01); G01N 33/56911 (2013.01); G01N 27/3275 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A kit for binding a target analyte, the kit comprising:
(a) an analyte probe comprising: (i) a specific binding pair member capable of specifically binding to a target analyte, and (ii) a detection moiety bound to the specific binding pair member; and
(b) a biosensor comprising (i) a non-specific binding pair member immobilized on a working electrode of the biosensor, the non-specific binding pair member comprising a carbohydrate moiety capable of non-specific binding to the target analyte, and (ii) nanoparticles for enhancing conductivity immobilized on the working electrode of the biosensor.