US 12,332,224 B2
Guard column configurations for size exclusion chromatography separations
Stephen James Shiner, Holden, MA (US); Frank J. Marszalkowski, Jr., Milford, MA (US); Steven M. Byrd, Ashland, MA (US); Raymond P. Fisk, Norton, MA (US); Mathew H. DeLano, Needham, MA (US); and Lavelay Kizekai, Coventry, RI (US)
Assigned to Waters Technolgies Corporation, Milford, MA (US)
Filed by Waters Technologies Corporation, Milford, MA (US)
Filed on Apr. 15, 2021, as Appl. No. 17/231,853.
Claims priority of provisional application 63/010,493, filed on Apr. 15, 2020.
Prior Publication US 2021/0325352 A1, Oct. 21, 2021
Int. Cl. B01D 15/34 (2006.01); B01D 15/22 (2006.01); G01N 30/08 (2006.01); G01N 30/46 (2006.01); G01N 30/60 (2006.01)
CPC G01N 30/606 (2013.01) [B01D 15/22 (2013.01); B01D 15/34 (2013.01); G01N 30/461 (2013.01); G01N 30/6065 (2013.01); G01N 2030/085 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A chromatographic system for size exclusion chromatography (SEC) comprising an inlet, an outlet, an analytic column having a first interior volume containing a first stationary phase, and a guard column having a second interior volume containing a secondary stationary phase, the inlet in fluid communication with the guard column, the guard column in fluid communication with the analytic column, and the analytic column in fluid communication with the outlet, wherein the first interior volume has a first length and a first cross-sectional area normal to the first length, wherein the second interior volume has a second length and a second cross-sectional area normal to the second length, wherein the second length is smaller than the first length and wherein the second cross-sectional area is smaller than the first cross-sectional area which ranges from 10 mm2 to 40 mm2, wherein the guard column has a diameter of 3.9 mm or less and is integrated into an inlet end of the analytical column and no ferrule connections are between the guard column and the inlet end of the analytical column.