US 12,332,222 B1
Liquid chromatography method for compounds having a sulfate group
Yury Zelechonok, Northbrook, IL (US)
Assigned to SIELC Technologies Corporation, Wheeling, IL (US)
Filed by Yury Zelechonok, Northbrook, IL (US)
Filed on Dec. 19, 2021, as Appl. No. 17/555,470.
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 30/34 (2006.01); B01D 15/08 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); G01N 30/02 (2006.01); G01N 30/88 (2006.01)
CPC G01N 30/34 (2013.01) [B01D 15/08 (2013.01); B01D 15/36 (2013.01); B01D 15/38 (2013.01); G01N 30/88 (2013.01); G01N 2030/027 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A method for separating compounds having a sulfate group using liquid chromatography employing a cation-exchange column and a mobile phase, the method comprising:
providing a mobile phase with an organic modifier and positively charged cations lacking more than one electron;
introducing into the mobile phase a sample containing the compounds having the sulfate group; and,
passing the mobile phase with the sample through the cation-exchange column containing a negatively charged stationary phase to separate out the compounds having a sulfate group.