| CPC G01N 21/78 (2013.01) [A01N 33/12 (2013.01); G01N 21/643 (2013.01); G01N 2021/6439 (2013.01)] | 8 Claims | 

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               1. A method for detecting coverage of an antimicrobial surface coating on a substrate, comprising the steps of: 
            providing a substrate including an antimicrobial surface coating; 
                applying an anionic agent to a surface of the substrate, wherein the anionic agent is an anionic thermochromic dye; 
                allowing the anionic agent to bond to the antimicrobial surface coating present on the substrate; 
                removing unbonded anionic agent from the surface of the substrate; 
                subjecting the surface of the substrate to a predetermined process to effect a change in the bonded anionic agent, wherein the predetermined process comprises applying heat from an external heat source to the surface of the substrate 
                observing the change in the bonded anionic agent, wherein the change observed in the bonded anionic agent is a visually detectable color change; and 
                determining, based on the observed change in the bonded anionic agent, coverage of the antimicrobial surface coating on the substrate. 
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