US 12,332,153 B2
Eosin staining techniques
Benjamin H. Blehm, Tucson, AZ (US); Evan T. Graves, Tucson, AZ (US); and Edward E. Durrant, Tucson, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Feb. 21, 2024, as Appl. No. 18/582,749.
Application 18/582,749 is a continuation of application No. 16/891,896, filed on Jun. 3, 2020, granted, now 11,940,358.
Application 16/891,896 is a continuation of application No. PCT/EP2018/086062, filed on Dec. 20, 2018.
Claims priority of provisional application 62/610,211, filed on Dec. 24, 2017.
Prior Publication US 2024/0272043 A1, Aug. 15, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 1/30 (2006.01)
CPC G01N 1/30 (2013.01) [G01N 2001/302 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of staining a specimen disposed on a substrate with an automated specimen processing apparatus, the method comprising:
(a) dispensing a predetermined amount of a primary staining solution to the specimen;
(b) at least partially removing the primary staining solution from the specimen;
(c) dispensing a predetermined amount of an alkaline solution to the specimen;
(d) at least partially removing the alkaline solution from the specimen;
(e) dispensing a predetermined amount of at least one additional solution to the specimen;
(f) at least partially removing the at least one additional solution from the specimen;
(g) dispensing a predetermined amount of a counter staining solution to the specimen;
(h) at least partially removing the counter staining solution from the specimen; and
(i) dispensing a predetermined amount of an acid solution to the specimen.