US 12,331,365 B2
Adenosine triphosphatase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2) gene-based molecular marker for identifying chicken feed efficiency trait, and identification method and use thereof
Sihua Jin, Hefei (CN); Yuanfei Ding, Hefei (CN); Zhaoyu Geng, Hefei (CN); Yi Fang, Hefei (CN); Xin Wang, Hefei (CN); Xiaoqing Hu, Hefei (CN); Xin Wang, Hefei (CN); Xuling Liu, Hefei (CN); Xing Liu, Hefei (CN); Yiran Luo, Hefei (CN); Lu Feng, Hefei (CN); and Dehua Zha, Hefei (CN)
Assigned to Anhui Agricultural University, Hefei (CN)
Filed by Anhui Agricultural University, Hefei (CN)
Filed on May 23, 2024, as Appl. No. 18/672,154.
Claims priority of application No. 202310596686.4 (CN), filed on May 23, 2023.
Prior Publication US 2025/0043365 A1, Feb. 6, 2025
Int. Cl. C12P 19/34 (2006.01); C12Q 1/44 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6888 (2018.01); G01N 27/447 (2006.01)
CPC C12Q 1/6888 (2013.01) [C12Q 1/44 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/686 (2013.01); G01N 27/44726 (2013.01); C12Q 2600/124 (2013.01); G01N 2333/916 (2013.01)] 3 Claims
 
1. A method for identifying a chicken feed efficiency trait using a molecular marker, comprising the following steps:
(1) extracting total DNA from venous blood of a chicken wing;
(2) designing specific amplification primers with a sequence comprising a locus at which the molecular marker is located and upstream and downstream bases thereof as a target sequence, and using the specific amplification primers to conduct PCR amplification with the total DNA as a template to obtain an amplification product;
(3) genotyping and sequencing the amplification product to obtain a molecular marker type of a chicken to be tested; and
(4) determining the chicken feed efficiency trait based on the molecular marker type; wherein
the molecular marker is an adenosine triphosphatase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2) gene-based molecular marker and has the sequence set forth in SEQ ID NO: 1; and wherein
if the molecular marker type of the chicken to be tested is a GG genotype, the chicken feed efficiency trait is at a high level;
if the molecular marker type of the chicken to be tested is a TT genotype, the chicken feed efficiency trait is at a low level;
if the molecular marker type of the chicken to be tested is a TG genotype, the chicken feed efficiency trait is at a medium level; and
the chicken to be tested is a Wannan three-yellow chicken, and the chicken feed efficiency trait is determined according to residual feed intake (RFI);
wherein sequences of the specific amplification primers are as follows:
 
 SEQ ID NO: 2:
 
 forward primer:
 
 TTTAGCAGCGATGGATGT;
 
 and
 
 
 
 SEQ ID NO: 3:
 
 reverse primer:
 
 GCGAGGAGGTATGAACTG.