US 12,331,353 B2
DNA concatemers on a surface
Radoje Drmanac, Los Altos Hills, CA (US); Matthew J. Callow, Redwood City, CA (US); Snezana Drmanac, Los Altos Hills, CA (US); Brian K. Hauser, Campbell, CA (US); and George Yeung, Mountain View, CA (US)
Assigned to Complete Genomics, Inc., San Jose, CA (US)
Filed by Complete Genomics, Inc, San Jose, CA (US)
Filed on Mar. 1, 2024, as Appl. No. 18/592,843.
Application 18/592,843 is a continuation of application No. 17/522,708, filed on Nov. 9, 2021.
Application 17/522,708 is a continuation of application No. 16/994,343, filed on Aug. 14, 2020, abandoned.
Application 16/994,343 is a continuation of application No. 16/425,846, filed on May 29, 2019, abandoned.
Application 16/425,846 is a continuation of application No. 15/442,659, filed on Feb. 25, 2017, granted, now 10,351,909, issued on Jul. 16, 2019.
Application 15/442,659 is a continuation of application No. 14/714,133, filed on May 15, 2015, granted, now 9,650,673, issued on May 16, 2017.
Application 14/714,133 is a continuation of application No. 12/882,880, filed on Sep. 15, 2010, abandoned.
Application 12/882,880 is a continuation of application No. 11/451,691, filed on Jun. 13, 2006, granted, now 8,445,194, issued on May 21, 2013.
Claims priority of provisional application 60/776,415, filed on Feb. 24, 2006.
Claims priority of provisional application 60/725,116, filed on Oct. 7, 2005.
Claims priority of provisional application 60/690,771, filed on Jun. 15, 2005.
Prior Publication US 2024/0263230 A1, Aug. 8, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6874 (2018.01); C07H 21/04 (2006.01); C07K 1/04 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/682 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6869 (2018.01); G01N 15/1404 (2024.01); G01N 15/1434 (2024.01)
CPC C12Q 1/6874 (2013.01) [C07H 21/04 (2013.01); C07K 1/047 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/682 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6869 (2013.01); G01N 15/1404 (2013.01); G01N 15/1434 (2013.01); C12Q 2525/151 (2013.01); C12Q 2525/313 (2013.01); C12Q 2531/125 (2013.01); C12Q 2565/513 (2013.01); Y10S 977/778 (2013.01); Y10S 977/789 (2013.01); Y10S 977/792 (2013.01); Y10S 977/88 (2013.01); Y10S 977/882 (2013.01)] 14 Claims
 
1. A method of characterizing polynucleotide molecules, said method comprising the steps of:
(a) providing a solid support comprising a surface;
(b) providing a plurality of polynucleotide molecules attached to the surface, wherein each polynucleotide molecule has a random coil state and comprises a concatemer of multiple copies of one or more target sequences,
wherein each polynucleotide molecule is attached to the surface within a binding region substantially equivalent to a projection of the random coil on the surface,
wherein the polynucleotide molecules are arranged on the surface such that the polynucleotide molecules can be resolved by optics of an imaging system; and then
(c) detecting complete or partial nucleotide sequences of said one or more target sequences from said polynucleotide molecules from images of said plurality of polynucleotide molecules obtained using said imaging system;
wherein the solid support is not a bead.