DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
Jingyue Ju, Englewood Cliffs, NJ (US); Dae Hyun Kim, Northbrook, IL (US); Jia Guo, Chandler, AZ (US); Qinglin Meng, Foster City, CA (US); Zengmin Li, Flushing, NY (US); and Huanyan Cao, Changzhou (CN)
Assigned to THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, New York, NY (US)
Filed by The Trustees of Columbia University in the City of New York, New York, NY (US)
Filed on Jan. 14, 2022, as Appl. No. 17/576,184.
Application 16/384,461 is a division of application No. 14/820,254, filed on Aug. 6, 2015, granted, now 10,260,094, issued on Apr. 16, 2019.
Application 17/576,184 is a continuation of application No. 16/384,461, filed on Apr. 15, 2019, granted, now 11,242,561.
Application 14/820,254 is a continuation of application No. 12/734,229, granted, now 9,115,163, issued on Aug. 25, 2015, previously published as PCT/US2008/011913, filed on Oct. 17, 2008.
Claims priority of provisional application 60/999,580, filed on Oct. 19, 2007.
Claims priority of provisional application 60/999,575, filed on Oct. 19, 2007.
Prior Publication US 2022/0290227 A1, Sep. 15, 2022
1. A deoxyribonucleic acid having attached at a 3′ end thereof, by a phosphodiester bond, a compound having the structure:
wherein the α labeled O atom is the 3′ O atom of the deoxyribonucleic acid, the wavy line represents the remainder of the deoxyribonucleic acid that is 5′ relative to the 3′O, and wherein R′ is a cleavable chemical group.
