US 12,331,348 B2
Detection probe for internal amplification control and kit including same
Miroslav Vranes, Hilden (DE); Ralf Peist, Hilden (DE); Mario Scherer, Hilden (DE); and Stefan Otto Cornelius, Hilden (DE)
Assigned to QIAGEN GmbH, Hilden (DE)
Filed by QIAGEN GmbH, Hilden (DE)
Filed on May 19, 2022, as Appl. No. 17/748,928.
Application 17/748,928 is a continuation of application No. 16/333,143, granted, now 11,345,952, issued on May 31, 2022, previously published as PCT/EP2017/073304, filed on Sep. 15, 2017.
Claims priority of application No. 16189225 (EP), filed on Sep. 16, 2016.
Prior Publication US 2022/0333176 A1, Oct. 20, 2022
Int. Cl. C12Q 1/6844 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/6851 (2018.01)
CPC C12Q 1/6846 (2013.01) [C12Q 1/6848 (2013.01); C12Q 1/6851 (2013.01); C12Q 2545/101 (2013.01); C12Q 2600/166 (2013.01)] 10 Claims
 
1. A kit comprising:
a set of primers having
a nucleotide sequence according to SEQ ID NO. 2 or a nucleotide sequence having at least the first fifteen 3′ nucleotides in common with the sequence according to SEQ ID NO. 2, and
a nucleotide sequence according to SEQ ID NO. 3 or a nucleotide sequence having at least the first fifteen 3′ nucleotides in common with the sequence according to SEQ ID NO. 3; and
an oligonucleotide probe selected from the group consisting of:
SEQ ID NO. 4;
a reverse complement of SEQ ID NO. 4;
an oligonucleotide that shares 95% sequence identity with SEQ ID NO. 4; and
an oligonucleotide that shares 95% sequence identity with a reverse complement of SEQ ID NO. 4.