	US 12,331,347 B2
	Methods for high-throughput labelling and detection of biological features in situ using microscopy
George M. Church, Brookline, MA (US); Je-Hyuk Lee, Allston, MA (US); and Evan R. Daugharthy, Cambridge, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Jan. 20, 2023, as Appl. No. 18/157,108.
Application 18/157,108 is a continuation of application No. 16/200,831, filed on Nov. 27, 2018, abandoned.
Application 16/200,831 is a continuation of application No. 15/325,577, granted, now 10,179,932, issued on Jan. 15, 2019, previously published as PCT/US2015/039914, filed on Jul. 10, 2015.
Claims priority of provisional application 62/023,226, filed on Jul. 11, 2014.
Prior Publication US 2023/0227895 A1, Jul. 20, 2023
Int. Cl. C12Q 1/6841 (2018.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01)

CPC C12Q 1/6841 (2013.01) [C07H 21/02 (2013.01); C07H 21/04 (2013.01)]

14 Claims

1. A method for processing a nucleic acid molecule in a cell, comprising:

(a) reverse transcribing an RNA molecule comprising a stem-loop structure in a cell, wherein said RNA molecule self-primes complementary DNA (cDNA) synthesis, thereby producing a first intermediate nucleic acid molecule comprising RNA, DNA, and said stem-loop structure;

(b) removing said stem-loop structure from said first intermediate nucleic acid molecule or derivative thereof to produce a single-stranded DNA molecule; and